Table 3.

Distribution of mutations in the rpoB RRDR of 109 rifampin-resistant and 49 rifampin-susceptible M. tuberculosis isolates from Nepal

Mutated codon(s)	Amino acid change(s)a	Nucleotide change(s)	No. (%) of isolates
			RIFr (n = 109)	RIFs (n = 49)
511	Leu→Pro	CTG→CCG	1 (0.9)	0 (0.0)
513	Gln→Leu	CAA→CTA	2 (1.8)	0 (0.0)
	Gln→Lys	CAA→AAA	2 (1.8)	0 (0.0)
514	Phe (ins)	TTC→TTCTTC	1 (0.9)	0 (0.0)
516	Asp→Val	GAC→GTC	13 (11.9)	0 (0.0)
	Asp→Phe	GAC→TTC	2 (1.8)	0 (0.0)
	Asp→Tyr	GAC→TAC	1 (0.9)	0 (0.0)
526	His→Tyr	CAC→TAC	5 (4.6)	0 (0.0)
	His→Arg	CAC→CGC	4 (3.7)	0 (0.0)
	His→Asp	CAC→GAC	3 (2.8)	0 (0.0)
	His→Cys	CAC→TGC	2 (1.8)	0 (0.0)
	His→Gly	CAC→GGC	1 (0.9)	0 (0.0)
	His→Leu	CAC→CTC	1 (0.9)	0 (0.0)
531	Ser→Leu	TCG→TTG	61 (56.0)	0 (0.0)
	Ser→Gln	TCG→CAG	1 (0.9)	0 (0.0)
	Ser→Val	TCG→GTG	1 (0.9)	0 (0.0)
533	Leu→Pro	CTG→CCG	2 (1.8)	0 (0.0)
531 and 514	Ser→Leu and Phe (ins)	TCG→TTG and TTC→TTCTTC	1 (0.9)	0 (0.0)
513 and 526	Gln→Lys and His→Asp	CAA→AAA and CAC→GAC	1 (0.9)	0 (0.0)
516 and 533	Asp→Ala and Leu→Pro	GAC→GCC and CTG→CCG	1 (0.9)	0 (0.0)
Wild typeb	None	None	3 (2.8)	49 (100.0)

^aPhe (ins), Phe insertion.

^bThat is, there were no mutations in the sequenced region.