Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s)a | Reference |
|---|---|---|
| Strains | ||
| Lactococcus lactis subsp. lactis | ||
| IL1403 | Indicator strain for GarML | 9a |
| IL1403 200B1 | GarML-resistant isolate | This study |
| IL1403 200C1 | GarML-resistant isolate | This study |
| IL1403 150G1 | GarML-resistant isolate | This study |
| IL1403 150G2 | GarML-resistant isolate | This study |
| IL1403 150H3 | GarML-resistant isolate | This study |
| IL1403 150H4 | GarML-resistant isolate | This study |
| Lactococcus garvieae DCC43 | Producer strain of GarML | 51 |
| Plasmids | ||
| pNZ8037 | Expression vector containing the nisin-inducible promoter PnisA; MCS; Camr | 12 |
| pNZ9530 | Lactococcal expression vector of nisRK; Ermr | 34 |
| pCG11 | Expression vector with malEFG fusion under the nisin-inducible promoter PnisA; Camr | This study |
a
Camr, chloramphenicol resistance; Tetr, tetracycline resistance; Ampr, ampicillin resistance; Ermr, erythromycin resistance; MCS, multiple-cloning site.