Table 1.
Primers used in this study
| Primers | Sequence (5′–3′) | Target |
|---|---|---|
| Real-time RT-PCR | ||
| TR AADA2 F | GCGATGAGCGAAATGTAGTG | aadA2 transcription |
| TR AADA2 R | GGCAGGTAGGCGTTTTATTG | |
| TR QNRVC F | CTTCTCACATCAGGACTTGCA | qnrVC1 transcription |
| TR QNRVC R | AATCGCACCCTTCCAATG | |
| TR RPOA F | GAACAAATCAGCACGACACA | V. cholerae rpoA transcription |
| TR RPOA R | CACAACCTGGCATTGAAGA | |
| TR AMP F | TTTATCCGCCTCCATCCA | bla transcription from the pGEM vector |
| TR AMP R | AGCCATACCAAACGACGAG | |
| Conventional PCR and sequencing | ||
| INT1P F | AAACCTTGCGCTCGTTC | Class 1 integron variable region |
| INB | AAGCAGACTTGACCT | |
| INF | GGCATCCAAGCAGCAAG | |
| M13 F | CGCCAGGGTTTTCCCAGTCACGAC | Cloned insert into pGEM vector |
| M13 R | TCACACAGGAAACAGCTATGAC | |
| T7 GFP Reverse | TAATACGACTCACTATAGGG GGGTAAGCTTTCCGTATGTAGC | Cloned insert into pGLOW |
| 5′ RACE | ||
| GSP1—QNRVC | CACAGCCTTGTACTCTAAAC | First-strand cDNA synthesis |
| GSP2—QNRVC | ACACCACGGCTTAAATCTGA | PCR for accessing the TSS |
| AUAP | GGCCACGCGTCGACTAGTAC | |
| Promoter regions (promoterless probe-vector) | ||
| pQL896-Pc F | GAGCTCGAATTCAAACCTTGCGCTCGTTC | Pc promoter region |
| pQL896-Pc R | CTGCAGAAGCTTGTTGCTGCTCCATAACATCA | |
| pGLOW-Pc F | AAACCTTGCGCTCGTTC | |
| pGLOW-Pc R | GCATACTGCAATCATCCTGTTCGGTCAAGGTTCTGGA | |
| pQL896-QNR F | GAGCTCGAATTCCCGGCGTTATGTGCTTTCT | Putative qnrVC promoter region |
| pQL896-QNR R | CTGCAGAAGCTTTGCAAGTCCTGATGTGAGAAG | |
| pGLOW-QNR F | TTGGCTAAAACGGGGTGT | |
| pGLOW-QNR R | GCATACTGCAATCATCCTCATGCTGTGGCTCCAAAA |