Fig 4.

Effect of serum on the antibacterial activity of OH-CATH30 and its analogs. (A) Peptides were preincubated with human serum for 0 to 12 h. Aliquots were taken at different time intervals, and the antibacterial activities of the aliquots against E. coli 25922 were determined using an agar radial diffusion method. (B) The antibacterial activity of the peptides was tested in the presence of 25% human serum using a microdilution assay. Each value shown is the mean ± standard error of results from three experiments.