Fig 2.
Effect of Lon on ciprofloxacin-mediated stimulation of mutagenesis. Cultures of P. aeruginosa wild-type strain H103, the lon mutant, and the complemented mutant (lonc) were diluted to an OD600 of 0.1 and spread onto an LB plate. A hole was punched in the middle of the agar plate, and it was filled with a total of 5 μg of ciprofloxacin. Plates were incubated at 37°C for 48 to 72 h, and the resistant colonies formed in the clearing zone were counted. Results from one of three representative experiments are shown.