Fig 3.

Effect of 6-NDA on S. cerevisiae transcription factor mutants. The parent strain (BY4742) and mutant strains harboring deletions in 14 different transcription factors genes were grown as described in Materials and Methods. Dilutions (5-fold) were prepared from each culture, inoculated onto YPD agar (MOPS buffered, pH 7.0), and incubated for 2 days at 30°C. Mutants known to have altered sensitivity to oleate (described in reference 23) are marked with asterisks. −6-NDA, medium containing solvent (DMSO); +6-NDA, medium containing 6-NDA at 4.5 μg/ml.