Fig 6.

Effect of 6-NDA on fluconazole activity in a clinical isolate of C. albicans. (A) A checkerboard assay was performed to evaluate the combined effects of 6-NDA and fluconazole in a fluconazole-resistant clinical isolate (isolate 17) of C. albicans (described in reference 52). Cells were grown in the presence of 3-fold serially diluted concentrations of 6-NDA and fluconazole for 48 h at 35°C, and OD readings were measured on a microplate reader. (B) To assess viability, 2-μl aliquots from each well were spotted on a fresh YPD agar plate, which was incubated for 24 h at 35°C.