Effects of ciprofloxacin (CIP), meropenem (MEM), azithromycin (AZN), rifaximin (RIF), tigecycline (TGC), and chloramphenicol (CHL) at 1/2 MIC on stx2 transcription in EHEC O104:H4 outbreak isolates LB226692 (A) and LB226806 (B) and EHEC O157:H7 strain EDL933 (C). Data show fold increases in stx2 transcription compared to that in antibiotic-free cultures and are means ± standard deviations for three independent experiments. *, P < 0.01 (unpaired Student's t test).