Fig 6.

Increase in soluble selectin, MCP-1, and IL-6 levels by consecutive doses of N9, SDS, and BZK. Mice were treated intravaginally with three consecutive doses of 40 mg/ml CMC, 10 mg/ml TFV, 40 mg/ml N9, 40 mg/ml SDS, and 20 mg/ml BZK in PBS or PBS alone. CVL fluid was collected at 4 h after each application and 28 and 52 h after the last application. Supernatant was separated from cell debris by centrifugation and was evaluated for detection of soluble factors by ELISA. Each dose administration is marked by a black arrow. (A and B) Responses of proinflammatory chemokine MCP-1 (A) and IL-6 (B) in mouse CVL fluid samples; (C to E) responses of soluble L-selectin (C), P-selectin (D), and E-selectin (E) in mouse CVL fluid samples. Results represent mean ± SD from five mice for one experiment. P values are for the proinflammatory compound treatment group versus PBS control at each time point: *, P < 0.05; **, P < 0.01; ***, P < 0.001.