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. 2012 Jun;56(6):3121–3132. doi: 10.1128/AAC.05950-11

Table 1.

Time points of composite mouse histopathology scores obtained after a single administration of 40 mg/ml N9 in PBS or PBS alone (as control)d

Time (h) Histopathology score
PBS
 N9
Total Epithelial cell disruption Leukocyte infiltration Edema Congestion Total Epithelial cell disruption Leukocyte infiltration Edema Congestion
0 2.0 ± 0.0 0.0 ± 0.0 1.0 ± 0.0 0.0 ± 0.0 1.0 ± 0.0 2.0 ± 0.0 0.0 ± 0.0 1.0 ± 0.0 0.0 ± 0.0 1.0 ± 0.0
2 2.2 ± 0.45 0.0 ± 0.0 1.2 ± 0.45 0.0 ± 0.0 1.0 ± 0.0 7.8 ± 1.48c 3.2 ± 0.45c 2.8 ± 0.45c 0.6 ± 0.55c 1.2 ± 0.45
4 2.8 ± 0.45 0.0 ± 0.0 1.8 ± 0.45 0.0 ± 0.0 1.0 ± 0.0 9.6 ± 0.89c 4.0 ± 0.0c 4.0 ± 0.0c 0.2 ± 0.45 1.4 ± 0.55
6 2.8 ± 0.45 0.0 ± 0.0 1.8 ± 0.45 0.0 ± 0.0 1.0 ± 0.0 8.4 ± 0.89c 4.0 ± 0.0c 2.8 ± 0.45b 0.0 ± 0.0 1.6 ± 0.55a
8 2.8 ± 0.84 0.0 ± 0.0 1.8 ± 0.84 0.0 ± 0.0 1.0 ± 0.0 7.4 ± 0.55c 4.0 ± 0.0c 2.2 ± 0.45 0.0 ± 0.0 1.2 ± 0.45
12 2.2 ± 0.45 0.0 ± 0.0 1.2 ± 0.45 0.0 ± 0.0 1.0 ± 0.0 6.4 ± 0.89c 3.4 ± 0.55c 1.8 ± 0.45 0.0 ± 0.0 1.2 ± 0.45
24 2.2 ± 0.45 0.0 ± 0.0 1.2 ± 0.45 0.0 ± 0.0 1.0 ± 0.0 5.0 ± 1.23c 2.4 ± 0.55c 1.4 ± 0.55 0.0 ± 0.0 1.2 ± 0.45
48 2.4 ± 0.55 0.0 ± 0.0 1.4 ± 0.55 0.0 ± 0.0 1.0 ± 0.0 3.0 ± 0.71 0.4 ± 0.55 1.6 ± 0.55 0.0 ± 0.0 1.0 ± 0.0
a

P < 0.05 compared with that of corresponding control group (two-way ANOVA, followed by Bonferroni posttests).

b

P < 0.01 compared with that of corresponding control group (two-way ANOVA, followed by Bonferroni posttests).

c

P < 0.001 compared with that of corresponding control group (two-way ANOVA, followed by Bonferroni posttests).

d

Mice were sacrificed at the indicated time points following a single application of 40 mg/ml N9 in PBS or PBS alone. The vaginal tissues were immediately taken and processed for histopathological examination, which was performed blindly by evaluation for epithelial cell disruption, leukocyte infiltration, edema, and congestion. Untreated mice were utilized as baseline controls, i.e., the 0-h time point, to document the normal tissue architecture and inflammation status in the vaginal mucosa. The histopathological scores were assigned by a semiquantitative system which was based on the scoring system of Eckstein et al. (11) and employed in our previous work (27). The detail of the scoring system is shown in Table S1 in the supplemental material. Data are represented as mean ± SD obtained from five mice per group of one experiment.