Table 1.
Diffusion times, their corresponding calculated diffusion coefficients, and fluorescence lifetimes for BODIPY and BODIPY-vancomycin in water and in the presence of S. aureus planktonic cellsa
| Method | Mean ± SEM or rangeb |
|||
|---|---|---|---|---|
| In water |
With S. aureus planktonic cells |
|||
| BODIPY | BODIPY-vancomycin | BODIPY | BODIPY-vancomycin | |
| FCS | ||||
| τ1 (μs) | 38 ± 6 (100) | 52 ± 11 (92) | 54 ± 9 (87) | 30,000–100,000 (100) |
| D1 (μm2/s) | 395 ± 60 | 290 ± 60 | 278 ± 46 | 0.15–0.50 |
| τ2 (ms) | 1.26 ± 0.62 (8) | 30–100 (13) | ||
| D2 (μm2/s) | 12 ± 6 | 0.15–0.50 | ||
| FLIM (ns) | 5.7 ± 0.2 | 5.7 ± 0.2 | 5.7 ± 0.2 | 4.5 ± 0.2 |
a
Diffusion times (τ1 and τ2) obtained from fitting with the equation from the fluorescent correlation curves, their corresponding calculated diffusion coefficients (D1 and D2), and the fluorescence lifetimes for BODIPY and BODIPY-vancomycin in water and in the presence of S. aureus plankton cells were determined. The percentages given in parentheses correspond to the ratio of molecules diffusing with the indicated time.
b
Values are expressed as means ± the SEM or as a range, where applicable. Percentages are indicated in parentheses where applicable.