Fig 1.

Phenotypic analysis of the NS5B S282T mutation in GT 1b, 1a, and JFH-1 2a replicons. Replicons containing S282T were generated by site-directed mutagenesis and tested for susceptibility to PSI-7977 using a 4-day transient assay. Cells containing GT 1a S282T/I434M replicons were also evaluated. (A) Replication fitness of S282T replicons from GT 1b, 1a, and JFH-1 2a relative to the corresponding wild type. (B) Effect of S282T on PSI-7977 activity. EC₅₀ fold change was determined by normalizing the EC₅₀s from the S282T replicons with that of the corresponding wild type. Results are reported as average ± SD from at least two independent experiments performed in duplicate.