Fig 2.

Iron-dependent expression of the hmuP gene. (A) HmuP or Irr protein in cells of the wild-type (Wt) or hmuP mutant was detected by Western blot analysis in cells grown in low-iron (L) or high-iron (H) medium. Thirty micrograms of protein was loaded per lane. (B) Analysis of hmuP mRNA by quantitative real-time PCR (qPCR) in wild-type or hmuP mutant cells grown in low- or high-iron medium. The data are expressed as the relative starting quantity (SQ) of hmuP mRNA normalized to the housekeeping gene gapA and are presented as the average of triplicate samples ± the standard deviation.