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In vivo occupancy of the hmuR-hmuP promoter by Irr in wild-type and hmuP mutant cells. Cells were grown in low-iron (L) or high-iron (H) medium, cross-linked, and immunoprecipitated with anti-Irr antibodies as described in the text. The precipitated DNA was analyzed by qPCR using primers that amplify promoter DNA from hmuR-hmuP, fegA, or bll5796. The data are expressed as the relative starting quantity (SQ) of immunoprecipitated DNA normalized to input DNA.
