Fig 6.

Iron-dependent expression of mRNA of genes within the heme utilization locus is affected in an hmuP mutant. hmuR, hmuT, fegA, and bll5796 mRNAs were analyzed by quantitative real-time PCR (qPCR) in wild-type (Wt) or hmuP mutant cells grown in low-iron (L) or high-iron (H) medium. The data are expressed as the relative starting quantity (SQ) The mRNAs were normalized to the housekeeping gene gapA, and the results are presented as the average of triplicate samples ± the standard deviation. Additional genes within the heme utilization locus are shown in Fig. S3 in the supplemental material.