Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or characteristic(s) | Reference or source |
|---|---|---|
| M. xanthus strains | ||
| DZ2 | Wild type | 9 |
| DK1622 | Wild type | 63 |
| DK101 | Wild type (sgl1) | 11 |
| PH1021 | DZ2 ΔmazF | This study |
| PH1022 | DZ2 Mxan_1658::pPH165 | This study |
| PH1023 | DK1622 ΔmazF | This study |
| PH1024 | DK101 ΔmazF | This study |
| PH1025 | DZ2 ΔmrpC | This study |
| PH1014 | DZ2 csgA::Tn5 lacΩLS205 | 13 |
| E. coli strains | ||
| TOP10 | Host for cloning [F−mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80lacZΔM15 ΔlacX74 deoR recA1 arsD139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupG] | Invitrogen |
| GJ1158 | proUp-T7 RNAP | 3 |
| Plasmids | ||
| pET28 | T7 promoter overexpression plasmid, Kmr | Novagen |
| pPH158 | pET28-mrpC | This study |
| pBJ114 | Backbone for in-frame deletions; galK Kmr | 16 |
| pPH165 | pBJ114 ΔmazF | This study |
| pPH164 | pBJ114 ΔmrpC | This study |