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TtpC2 within the TtpC2-TonB2 system is essential for vulnibactin uptake. Liquid chrome azurol S (CAS) assays were used to determine the relative production of V. vulnificus native siderophore vulnibactin. Supernatant from log phase cultures grown in minimal media were mixed with CAS solution at a 1:1 ratio. The OD630 was determined after 20 min of incubation at room temperature. Samples were normalized to WT.
