Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or relevant characteristica | Reference or source |
|---|---|---|
| Strains | ||
| V. vulnificus | ||
| CMCP6 | Wild type | J. Rhee |
| AA-14 | ΔvenB | 2 |
| AA-6 | ΔtonB1 | 2 |
| AA-7 | ΔtonB2 | 2 |
| VSRK301 | ΔttpC2 | This study |
| AA-8 | ΔtonB3 | 2 |
| VSRK198 | ΔttpC3 | This study |
| AA-9 | ΔtonB1 ΔtonB2 | 2 |
| VSRK357 | ΔtonB1 ΔttpC2 | This study |
| AA-10 | ΔtonB1 ΔtonB3 | 2 |
| VSRK199 | ΔtonB1 ΔttpC3 | This study |
| AA-11 | ΔtonB2 ΔtonB3 | 2 |
| VSRK275 | ΔttpC2 ΔttpC3 | This study |
| AA-12 | ΔtonB1 ΔtonB2 ΔtonB3 ΔlacZ | 2 |
| VSRK202 | ΔtonB1 ΔtonB2 ΔttpC3 | This study |
| VSRK304 | ΔtonB1 ΔttpC2 ΔttpC3 | This study |
| AA-16 | ΔtonB1 ΔtonB2 ΔtonB3 ΔvenB | 2 |
| VSRK283 | ΔtonB1 ΔttpC2 ΔttpC3 ΔvenB | This study |
| V. parahaemolyticus | ||
| RIMD | Wild type | T. Honda |
| E. coli | ||
| S17-1 λpir | λpir lysogen; thi pro hsdR hsdM+recA RP4-2 Tc∷Mu Km∷Tn7; Tpr Smr | 46 |
| Plasmids | ||
| pCR2.1 | TA cloning vector; Kmr Ampr | Invitrogen |
| pMMB208 | Broad-host-range expression vector; CmrPtac | 36 |
| pDM4 | Suicide vector with oriR6K; CmrsacB | 35 |
| pRK2013 | Helper plasmid; Kmr | 15 |
| ptonB1 | V. vulnificus CMCP6 tonB1 cloned into pMMB208 | 2 |
| ptonB2 | V. vulnificus CMCP6 tonB2 cloned into pMMB208 | 2 |
| pttpC2(Vvul) | V. vulnificus CMCP6 ttpC2 cloned into pMMB208 | This study |
| pttpC2(Vcho) | V. cholerae CA401 ttpC2 cloned into pMMB208 | This study |
| pttpC2(Vpara) | V. parahaemolyticus RIMD ttpC2 cloned into pMMB208 | This study |
| pttpC2(Valg) | V. alginolyticus 12G01 ttpC2 cloned into pMMB208 | This study |
| pttpC2(Vang) | V. anguillarum 775 ttpC2 cloned into pMMB208 | This study |
a
Ampr, ampicillin resistant; Cmr, chloramphenicol resistant; Kmr, kanamycin resistant; Smr, streptomycin resistant; Tpr, trimethoprim resistant.