Table 3.
TtpC2 is an essential component of the TtpC2-TonB2 system in mediating the uptake of exogenous siderophores in V. vulnificus
| V. vulnificus strain or genotype | Growth on indicated iron sourcea |
||||||
|---|---|---|---|---|---|---|---|
| FAC | Vibriobactin | Vibrioferrin | Ferrioxamine | Aerobactin | Enterobactin | Anguibactin | |
| WT | + | + | + | + | + | + | − |
| ΔtonB1 | + | + | + | + | + | + | − |
| ΔtonB2 | + | + | + | + | − | + | − |
| ΔttpC2 | + | + | + | + | − | + | − |
| ΔtonB3 | + | + | + | + | + | + | − |
| ΔttpC3 | + | + | + | + | + | + | − |
| ΔtonB1 ΔtonB2 | + | − | − | − | − | − | − |
| ΔtonB1 ΔttpC2 | + | − | − | − | − | − | − |
| ΔtonB1 ΔtonB3 | + | + | + | + | + | + | − |
| ΔtonB1 ΔttpC3 | + | + | + | + | + | + | − |
| ΔtonB2 ΔtonB3 | + | + | + | + | − | + | − |
| ΔtonB1 ΔtonB2 ΔtonB3 | + | − | − | − | − | − | − |
| ΔtonB1 ΔttpC2 ΔttpC3 | + | − | − | − | − | − | − |
| ΔtonB1 ΔttpC2 ΔttpC3 ΔvenB/pMMB208 | + | − | − | − | − | − | − |
| ΔtonB1 ΔttpC2 ΔttpC3 ΔvenB/ptonB1 | + | + | + | + | − | + | − |
| ΔtonB1 ΔtonB2 ΔttpC3 ΔvenB/ptonB2 | + | + | + | + | + | + | − |
| ΔtonB1 ΔttpC2 ΔttpC3 ΔvenB/pttpC2(Vvul) | + | + | + | + | + | + | − |
a
Growth was determined by the presence of a halo (+) or lack thereof (−) around the iron source indicated. Two microliters of each iron source was spotted on the surface of the plates in the following concentrations: FAC, 500 μg/ml; vibriobactin, 1.0 mg/ml; anguibactin, 1.0 mg/ml; aerobactin, 1.0 mg/ml; enterobactin, 1.0 mg/ml; and ferrioxamine, 1.0 mg/ml. Wild-type V. parahaemolyticus producing vibrioferrin was streaked onto the plate, and the halo of growth was monitored around the streak.