Table 4.

Complementation of ttpC2 from other Vibrio species restores the TtpC2-TonB2-mediated uptake of various iron sources^a

Iron sourceb	TtpC from other Vibrio species containing the indicated complementing plasmid
	pMMB208	pttpC2(Vvul)	pttpC2(Vcho)	pttpC2(Vpar)	pttpC2(Valg)	pttpC2(Vang)
FAC	+	+	+	+	+	+
Vulnibactin	−	+	+	+	+	+
Hydroxamate	−	+	+	+	+	+
Vibriobactin	−	+	+	+	+	+
Vibrioferrin	−	+	+	+	+	+
Aerobactin	−	+	+	+	+	+
Anguibactin	−	−	−	−	−	−
Enterobactinc	−	+	+	+	+	+
Ferrichrome	−	−	−	−	−	−
Ferrioxamine	−	+	+	+	+	+

^aThe embedded strain VSRK283 (ΔtonB1 ΔttpC2 ΔttpC3 ΔvenB) contained the complementing plasmid pMMB208 expressing TtpC2 from the Vibrio species containing the plasmids listed at the top of the table.

^bGrowth was determined by the presence of a halo (+) or lack thereof (−) around the iron source indicated. Two microliters of each iron source was spotted on the surface of the plates in the following concentrations: FAC, 500 μg/ml; vibriobactin, 1.0 mg/ml; aerobactin, 1.0 mg/ml; anguibactin, 1.0 mg/ml; enterobactin, 1.0 mg/ml; ferrichrome, 1.0 mg/ml; and ferrioxamine, 1.0 mg/ml. Wild-type V. vulnificus and V. parahaemolyticus producing vulnibactin and vibrioferrin, respectively, were streaked onto the plate, and the halo of growth was monitored around the streak. A V. vulnificus ΔvenB mutant was streaked onto the plate to test for growth around the hydroxamate siderophore.

^cGrowth around enterobactin was only seen when the imbedded strain being tested was grown in a minimal medium CM9 plate and not in a rich medium TSAS plate.