Fig 2.

Outer membrane protein biogenesis. (A) Steady-state levels of various proteins in the outer membrane fraction of indicated mutant strains. Pronounced effects are marked by red boxes. Braun's outer membrane lipoprotein (Lpp) served as a loading control. (B) mRNA levels of fhuA, lamB, and ompW were compared in wt and mutant strains by RT-qPCR as described in Table 3. Standard deviation was below 40%. For comparative and quantitative analysis, transcript levels were normalized to the level of gapA. (C) FhuA levels in various mutant strains. The comparison of FhuA levels in degP ppiD mutants with those in degP ppiD tsp mutants suggests that Tsp degrades FhuA in degP ppiD strains.