Table 3.
Induction of rpoE and spy genes in folding factor mutantsa
| Strain or relevant genotype | Fold induction (mean normalized expression) of: |
|
|---|---|---|
| rpoE | spy | |
| BW30270 (wt) | 1.0 | 1.0 |
| surA | 5.0 | 2.8 |
| dsbA | 2.6 | 6.0 |
| degP | 1.1 | 0.9 |
| tsp | 1.3 | 1.4 |
| dsbC | 1.4 | 1.0 |
| fkpA | 1.2 | 1.3 |
| ppiD | 1.2 | 1.0 |
| ydgD | 0.7 | 0.9 |
| yfgC | 1.0 | 0.9 |
| surA dsbC | 3.2 | 6.0 |
| dsbA fkpA | 3.3 | 11.2 |
| degP dsbA | 3.5 | 7.7 |
| degP tsp | 2.3 | 12.9 |
| tsp fkpA | 1.1 | 2.5 |
| tsp ydgD | 2.3 | 4.3 |
| tsp ppiD | 1.8 | 4.9 |
| degP ppiD | 0.8 | 0.9 |
| degP yfgC | 1.2 | 1.1 |
a
Cells were grown to mid-log phase in liquid minimal medium at 37°C. RNA levels of rpoE and spy were determined by RT-qPCR. Assays were done with two biological and three statistical replicates, and standard deviations were <40%.