Fig 2.

Interruption of the ECA synthesis pathway results in hypervesiculation. The amount of Kdo released from stationary-phase cells increased upon mutation of wecG. Reintroduction of wecG restored wild-type levels of vesiculation. Average Kdo values were calculated from five separate experiments, and the error bars indicate standard deviations. Also shown is TEM analysis of vesicles (indicated by arrows) in WT (inset A), wecG (inset B), and wecG (inset C) samples at 37°C. The scale bar present at the bottom left of each image corresponds to 200 nm.