Fig 4.

The loss of rcsB within an ECA mutant strain results in the reversion of the hypervesiculation phenotype. A comparison of the net amount of OMV recovered from the various strains is displayed in panel A. Panels B (37°C) and C (30°C) display the same data in terms of a fold difference in vesiculation,with WT levels set to 1. Note the difference in scale between panels B and C. These results demonstrate that introduction of a mutation into the rcsB gene reverts the hypervesiculation phenotype caused by the wecD mutation at 37°C. Average Kdo values were calculated from five separate experiments, and the error bars indicate standard deviations.