Table 1.
Proteins detected in the OM and OMVs of wild-type S. marcescensa
Fraction | Systematic gene name | Annotation | MW | Score |
---|---|---|---|---|
OMV | 4161L | Lipase | 64.9 | 1,387 |
6253L | Flagellin | 34.6 | 1,103 | |
12577R | Serralysin A | 54 | 879 | |
2860L | OmpF | 41.7 | 719 | |
2408R | Surface layer protein A | 101.2 | 639 | |
2927L | OmpA | 39.2 | 439 | |
7297L | Alanine amidase | 28.4 | 351 | |
6014L | Porin | 39.6 | 227 | |
4103L | Lipoprotein precursor | 8.3 | 147 | |
7646L | OmpC | 41.4 | 161 | |
5558L | OmpW | 23.8 | 139 | |
6463R | Hcp effector | 16.8 | 138 | |
5731L | MipA protein | 28.5 | 127 | |
1470R | Peptidoglycan-associated lipoprotein | 19.4 | 123 | |
12753R | EF-Tu | 43.2 | 110 | |
2143R | OmpX | 18.4 | 110 | |
8985R | Phospholipase | 35.6 | 103 | |
5653R | Alcohol dehydrogenase | 96.2 | 86 | |
OM | 2927L | OmpA | 39.2 | 1,494 |
2860L | OmpF | 41.7 | 1,034 | |
13014R | GroEL | 56.6 | 895 | |
6253L | Flagellin | 36.6 | 893 | |
7646L | OmpC | 41.4 | 832 | |
6014L | Porin | 39.6 | 498 | |
5558L | OmpW | 23.8 | 443 | |
1470R | Peptidoglycan-associated lipoprotein | 19.4 | 434 | |
6011L | LysM | 34.5 | 426 | |
5653R | Alcohol dehydrogenase | 96.2 | 329 | |
4176L | OM lipoprotein | 16.6 | 297 | |
4103L | Lipoprotein precursor | 8.3 | 292 | |
10953L | Ribosomal 30S | 23.6 | 220 | |
9153L | Omp factor YaeT | 89.6 | 193 | |
10162R | TolC | 54.5 | 190 | |
109L | LPS assembly protein LptD | 89.3 | 187 | |
6322L | Flagellar hook | 42.6 | 187 | |
12753R | EF-Tu | 43.2 | 165 | |
2143R | OmpX | 18.4 | 145 | |
2408R | Surface layer protein A | 101.2 | 141 | |
12670R | Ribosomal 50S | 24.6 | 124 | |
10954L | Ribosomal 30S | 17.6 | 122 | |
11000L | Ribosomal S7 | 13.9 | 117 | |
9916L | Dihydrolipoamide dehydrogenase | 43.9 | 107 |
Proteins detected by matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS). The score contributed to protein hits can also be used to estimate their prevalence within the sample. In bold, extracellular or hypothesized extracellular proteins are identified. MW, molecular weight.