Table 2.
Proteins detected in the OM and OMVs of WecD mutant strains of S. marcescensa
| Fraction | Systematic gene name | Annotation | MW | Score |
|---|---|---|---|---|
| OMV | 6253L | Flagellin | 34.6 | 920 |
| 5558L | OmpW | 23.8 | 697 | |
| 12753R | Ef-Tu | 43.2 | 624 | |
| 4161L | Lipase | 64.9 | 622 | |
| 10801L | Maltoporin | 48.2 | 596 | |
| 7646L | OmpC | 41.4 | 546 | |
| 6014L | Porin | 39.6 | 533 | |
| 8985R | Phospholipase | 35.6 | 523 | |
| 12577R | Serralysin A | 54.0 | 519 | |
| 5653R | Alcohol dehydrogenase | 96.2 | 317 | |
| 2860L | OmpF | 41.7 | 350 | |
| 2408R | SLA | 101.2 | 326 | |
| 7297L | Alanine amidase | 28.4 | 305 | |
| 12364R | Chitinase | 61.0 | 273 | |
| 5731L | MipA protein | 28.5 | 273 | |
| 1470R | Peptidoglycan-associated lipoprotein | 19.4 | 259 | |
| 9153L | Omp factor YaeT | 89.6 | 260 | |
| 2927L | OmpA | 39.2 | 250 | |
| 13014R | GroEL | 56.6 | 249 | |
| 3763R | Tsx | 33.4 | 234 | |
| 1467R | TolB | 46.0 | 227 | |
| 4103L | Lipoprotein precursor | 8.3 | 198 | |
| 10350R | Hypothetical protein | 19.9 | 191 | |
| 409R | Lipoprotein NlpD | 35.0 | 185 | |
| OM | 4161L | Lipase | 64.9 | 1,002 |
| 6253L | Flagellin | 34.6 | 929 | |
| 12577R | Serralysin A | 54 | 879 | |
| 2927L | OmpA | 39.2 | 798 | |
| 1467R | TolB | 46.0 | 629 | |
| 10814R | Putative membrane protein | 56.9 | 597 | |
| 7646L | OmpC | 41.4 | 537 | |
| 2408R | SLA | 101.2 | 530 | |
| 8612L | OM autotransporter | 102.3 | 476 | |
| 7297L | Alanine amidase | 28.4 | 474 | |
| 1283L | Glutamate transporter | 33.1 | 418 | |
| 2860L | OmpF | 41.7 | 354 | |
| 9153L | OM assembly protein | 89.6 | 349 | |
| 960L | Putative lipoprotein | 20.7 | 339 | |
| 5558L | OmpW | 23.8 | 334 | |
| 10801L | Maltoporin | 48.2 | 298 | |
| 1013R | Putative lipoprotein | 16.9 | 291 | |
| 2143R | OmpX | 18.4 | 289 | |
| 3535L | LolB | 25.0 | 281 | |
| 4561R | Serralysin B | 57.7 | 270 | |
| 4103L | Lipoprotein precursor | 8.3 | 268 | |
| 11524L | SSph2 | 107.5 | 261 | |
| 3763R | Tsx | 33.4 | 237 | |
| 5731L | MipA protein | 28.5 | 231 | |
| 9153L | Omp factor YaeT | 89.6 | 220 | |
| 6463R | Hcp1 | 16.8 | 213 | |
| 5643L | Periplasmic oligopeptide-binding protein | 61.5 | 204 | |
| 1473R | Tol-Pal protein YpgF | 28.1 | 201 | |
| 409R | Lipoprotein NlpD | 35.0 | 196 | |
| 3237L | YceI | 21.3 | 190 | |
| 1470R | Peptidoglycan-associated lipoprotein | 19.4 | 164 | |
| 4176L | OM lipoprotein | 16.6 | 162 | |
| 4456R | Peptidyl-dipeptidase Dcp | 82.4 | 156 | |
| 6014L | Porin | 39.6 | 151 | |
| 6011L | LysM | 34.5 | 151 | |
| 10162R | TolC | 54.5 | 150 | |
| 12369R | Extracellular solute-binding protein | 64.5 | 142 |
a
In bold, extracellular or hypothesized extracellular proteins are identified. Only proteins scoring above 100 are listed. The S. marcescens Db11 genome can be found at http://www.sanger.ac.uk/Projects/S_marcescens/. MW, molecular weight.