Fig 4.

Synthetic sick phenotype of ftsZ84 (Ts) cells in the absence of zapD. (A) Overnight cultures of wild-type (wt; TB28), zapD (JD310), ftsZ84 (Ts) (EC307), and ftsZ84 (Ts) zapD (JD330) cells grown in LB at 30°C were normalized to an OD₆₀₀ of 2.0 and serially diluted, and 6-μl aliquots were spotted on LB no-salt agar plates and incubated overnight at 30°C. The relative plating efficiencies were determined by comparison with control strains. (B and C) Phase images of ftsZ84 (Ts) (EC307) and ftsZ84 (Ts) zapD (JD330) cells. Overnight cultures grown in LB at 30°C were subcultured at 1:100 to an OD₆₀₀ of 0.6 to 0.8. Samples were immobilized on agarose pads and visualized by microscopy. Bar, 5 μm.