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ZapD promotes FtsZ-polymer bundling. (A) Negative stained TEM images of FtsZ (5 μM) in buffer containing MOPS (pH 6.5), 50 mM KCl, and 2.5 mM MgCl2 in the presence of 1 mM GTP show characteristic single protofilaments. (B) Upon addition of ZapD (5 μM), large bundled FtsZ protofilaments were visualized. Bar, 200 nm.
