Table 3.
Synergism of zapD with known Z-associated (Zap) proteins
| Genotypea | Cell length (μm ± SD) | No. of cells measuredb |
|---|---|---|
| Wild type | 3.4 ± 1.1 | 604 |
| zapA | 4.0 ± 1.9 | 605 |
| zapC | 3.9 ± 1.6 | 601 |
| zapD | 3.2 ± 0.9 | 601 |
| zapA zapD | 5.2 ± 3.7 | 602 |
| zapA zapD pNG162-zapDc | 4.2 ± 2.1 | 640 |
| zapC zapD | 4.2 ± 1.3 | 618 |
| zapA zapC | 6.2 ± 5.2 | 545 |
| zapA zapC zapD | 7.0 ± 6.3 | 625 |
The wild-type strain was TB28, and isogenic single (zapA = JD257; zapC = JD317; zapD = JD310)-, double (zapA zapC = JD305; zapA zapD = JD315; zapC zapD = JD345)-, and triple (zapA zapC zapD = JD360)-mutant strains were constructed by P1 transduction.
Data represent total numbers of cells measured for length using ObjectJ (NIH). Cells were grown in LB broth at 30°C to an OD600 of 0.6 to 0.8, at which point samples were immobilized on agarose pads and visualized by phase microscopy.
Strain AJ18-79 was subcultured at a dilution of 1:100 in LB with IPTG added to reach a 50 μM final concentration at 30°C and grown to an OD600 of 0.6 to 0.8, at which point cells were visualized by phase microscopy.