Table 1. Fine mapping markers.
Marker | Forward Primer | Reverse Primer | Tm (°C) |
---|---|---|---|
BMS1509192 | GAAGCAACCTAAGTGTCCATCA | ATCCATGTTTTTGCAAGTGG | TD |
BMS1509996 | TGTGAAGAGGAAAAGGCATCA | CTCCTCACGGGCAAGTAAGT | TD |
BMS1510292 | CGCAGCGAGGAAAATGTTTA | GGTCAGAATGACCTGGTCTCA | TD |
BMS1512327 | TTTGCCCAAGAGATGGATTG | GCCTGGAAGACTACGGTCTG | TD |
BMS1512786 | GATGGGAGAGCTGGAGACTG | CCTGGACTCTGCTCATCAAA | TD |
BMS1512958 | TGTATTTTGGGGGATGGAAA | ACATGCCTTGAGGCCAAA | TD |
BMS1513060 | GGAGAAAATTGAGGCAGCTT | CTGCAGCTCTGCAAAACCTA | TD |
BMS1513944 | GCACTGCGCTATTGGTGTTA | TTGTGGGGACACAGTTCAAA | TD |
T3S9 | TTTCCAGGCACTTTGTTTCC | AGGGCTTATAGGCGTGATGA | 64.3 |
T4S11 | CACTTCTGTGCTGCTGTGGT | TCAATCATCCTCCAAACACAA | 61.2 |
T6S8 | TGTGAAAGATGTCACAAATCACTG | GGAAGATTCCCTGGAGAAGG | 57 |
c.989G>A | CCTTGCCTTAGAAGCTGGAG | GCAGCCAGTCTCAGAGTCCT | 59 |
Primers and annealing temperatures for novel OAR chromosome 6 markers are shown. TD indicates a touchdown PCR, described in the marker genotyping section of the Methods.