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. 2012 May 25;68(Pt 6):724–729. doi: 10.1107/S1744309112015230

Figure 3.

Figure 3

X-ray crystallography. (a) Crystals from the initial hit for the E. coli O157 ParE2–PaaA2 complex similar in size to those used for microseeding. The black bar indicates the size of the crystals and corresponds to 500 µm. (b) Typical crystals of the E. coli O157 ParE2–PaaA2 complex prepared by microseeding and pictured on the same scale as in (a). (c) SDS–PAGE analysis of crystals of the E. coli O157 ParE2–PaaA2 complex. Lane M, Pre-stained Molecular Weight Marker; lane 1, sample of the purified ParE2–PaaA2 complex (c.f. Fig. 1 ▶); lane 2, sample of a washed and redissolved crystal; lane 3, sample of the mother liquor surrounding the crystal. (d) Diffraction pattern of a crystal similar in size to that shown in (b) and from which the data set reported in Table 1 ▶ was collected. It shows diffraction spots up to 3.8 Å resolution (the 3.8 Å resolution limit is indicated by an orange circle). The dashed box displays an enlargement of the diffraction pattern close to the 3.8 Å resolution limit.