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. 2012 May 21;3(6):1455–1464. doi: 10.1364/BOE.3.001455

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©2012 Optical Society of America

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.

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Fig. 3 — In vitro imaging of flowing blood cells. (a) SEFC raw image of diluted whole blood. Right insets, four individual RBCs of different orientations in space whose location in the raw image is marked by arrows. (b) Typical SEFC images of live granulocytes and lymphocytes separated from whole blood compared to standard fluorescence microscopy. Cells' nuclei were stained blue (Hoechst). The lymphocyte SEFC image was brightened (3×) to allow visual comparison. (c) Diameter histograms of granulocytes and lymphocytes, with corresponding mean ± S.D values. (d) Scatter plot of WBC as function of size and integrated brightness allows good statistical separation of lymphocytes from granulocytes.