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. 2012 May 21;3(6):1455–1464. doi: 10.1364/BOE.3.001455

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©2012 Optical Society of America

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.

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Fig. 6 — In vivo imaging of WBCs. (a) Imaging close to the wall of a post capillary venule allows forming a plot of the total intensity across the image as a function of time. Good correlation between the appearances of WBCs (magnified cell images below the plot) and intensity peaks was obtained. (b) Averaged flux of WBCs for different vessel diameters. (c) Diameter histogram of the imaged WBCs. (d) A single rolling WBC (outlined by a dashed blue line) near a vessel wall, characterized by an elongated shape which indicates its low relative velocity (approximately 0.18 mm/s) in contrast to the nearby fast RBC flow (approximately 1.1 mm/s). (e) Two attached WBCs flowing in a capillary. (f) A single WBC flowing in a small capillary with a downstream plasma gap (RBC depleted region).