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. Author manuscript; available in PMC: 2013 May 15.
Published in final edited form as: Dev Biol. 2012 Mar 3;365(2):339–349. doi: 10.1016/j.ydbio.2012.02.031

Figure 3.

Figure 3

Epidermal cells do not contribute to the blastema or change fate during regeneration. (A–L) Longitudinal sections of the caudal fin of Tg(dusp6:Cre-ERT2, EAB:EGFP-FlEx-mCherry) Class 1 mosaic animals demonstrating mCherry expression (red, A, E, I) and stained with anti-p63 antibodies to mark epidermal cells (green, B, F, J) and with Hoechst to mark nuclei (blue, C, G, K). The three-color overlays are shown in (D, H, L). (A–D) mCherry+ epidermal cells in Tg(dusp6:Cre-ERT2, EAB:EGFP-FlEx-mCherry) Class 1 mosaic animals remain positive for the epidermal maker p63 at 2 dpa. The yellow dashed line marks the approximate amputation site and the white dashed line marks the boundary between the epidermis and the blastema. (E–L) Stained fin sections from the same Tg(dusp6:Cre-ERT2, EAB:EGFP-FlEx-mCherry) Class 1 mosaic animal prior to amputation (E–H) and 7 dpa (I–L). White arrows indicate mCherry+/p63+ epidermis.