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. 2012 Jun 8;7(6):e38718. doi: 10.1371/journal.pone.0038718

Figure 4. Southern blot analysis of transgenic tobacco plants.

Figure 4

(A) HTG7; (B) AM79; (C) A1501; (D) RD; (E) G2. Five transgenic lines each construct were analyzed. 100 µg genomic DNA was digested with HindIII which has only one site in the plasmid, so the band numbers are equal to the copy number of transgene.