Table 1. Plasmids used in this study.
Plasmid | Relevant plasmid genotype | Reference |
pUChSA-1 | pUC19-SA1cDNA | J-L. Barbero, Centro de Investigaciones Biologicas (CSIC), Madrid, Spain |
pUChSA-2 | pUC18-SA2cDNA | as above |
pUG35 | PMET25- MCS-GFP-TCYC1 URA3 CEN | J. H. Hegemann, Heinrich-Heine-Universitat, Dusseldorf, Germany |
pIGoutA | PGAL1-1-67NLSH2B-2xGFP URA3 2 µ | based on pRS426(Clontech) |
pPK1 | PMET25–SA1–GFP–TCYC1 URA3 CEN | This study, based on pUG35 |
pLT1 | PMET25–SA2–GFP–TCYC1 URA3 CEN | This study, based on pUG35 |
pLT1-M1 | PMET25–SA2V698S–GFP–TCYC1 URA3 CEN | This study |
pLT1-M2 | PMET25-SA2F959E–GFP–TCYC1 URA3 CEN | This study |
pLT3 | PMET25–1-67NLSH2B–STAG2–GFP–TCYC1 URA3 CEN | This study |
pPK2 | PMET25–NLSSA1–GFP–TCYC1 URA3 CEN | This study |
pSA1 | PCMV-SA1-GFP-SV40 Late poly(A) | based on pCl-neo(Promega) |
pSA2L | PCMV-SA2L-GFP-SV40 Late poly(A) | as above |
pSA2S | PCMV-SA2S-GFP-SV40 Late poly(A) | as above |
pSA1Δ34–53 | PCMV-SA1ΔK34-53K-GFP-SV40 Late poly(A) | as above |
pSA2LΔ32–47 | PCMV-SA2ΔK32-47K-GFP-SV40 Late poly(A) | as above |
pSA2LΔC161 | PCMV-SA2ΔC161-GFP-SV40 Late poly(A) | as above |
pSA2LΔ1071–1140 | PCMV-SA2ΔR1071-S1140-GFP-SV40 Late poly(A) | as above |
pSA2Δ1199–1206 | PCMV- SA2ΔP1199-E1206 -GFP-SV40 Late poly(A) | as above |
Abbreviations for description of plasmids: CEN, centromeric; 2 µ, episomal; MCS, multiple cloning site.