Table 1. Plasmids used in this study.

Plasmid	Relevant plasmid genotype	Reference
pUChSA-1	pUC19-SA1cDNA	J-L. Barbero, Centro de Investigaciones Biologicas (CSIC), Madrid, Spain
pUChSA-2	pUC18-SA2cDNA	as above
pUG35	PMET25- MCS-GFP-TCYC1 URA3 CEN	J. H. Hegemann, Heinrich-Heine-Universitat, Dusseldorf, Germany
pIGoutA	PGAL1-1-67NLSH2B-2xGFP URA3 2 µ	based on pRS426(Clontech)
pPK1	PMET25–SA1–GFP–TCYC1 URA3 CEN	This study, based on pUG35
pLT1	PMET25–SA2–GFP–TCYC1 URA3 CEN	This study, based on pUG35
pLT1-M1	PMET25–SA2V698S–GFP–TCYC1 URA3 CEN	This study
pLT1-M2	PMET25-SA2F959E–GFP–TCYC1 URA3 CEN	This study
pLT3	PMET25–1-67NLSH2B–STAG2–GFP–TCYC1 URA3 CEN	This study
pPK2	PMET25–NLSSA1–GFP–TCYC1 URA3 CEN	This study
pSA1	PCMV-SA1-GFP-SV40 Late poly(A)	based on pCl-neo(Promega)
pSA2L	PCMV-SA2L-GFP-SV40 Late poly(A)	as above
pSA2S	PCMV-SA2S-GFP-SV40 Late poly(A)	as above
pSA1Δ34–53	PCMV-SA1ΔK34-53K-GFP-SV40 Late poly(A)	as above
pSA2LΔ32–47	PCMV-SA2ΔK32-47K-GFP-SV40 Late poly(A)	as above
pSA2LΔC161	PCMV-SA2ΔC161-GFP-SV40 Late poly(A)	as above
pSA2LΔ1071–1140	PCMV-SA2ΔR1071-S1140-GFP-SV40 Late poly(A)	as above
pSA2Δ1199–1206	PCMV- SA2ΔP1199-E1206 -GFP-SV40 Late poly(A)	as above

Abbreviations for description of plasmids: CEN, centromeric; 2 µ, episomal; MCS, multiple cloning site.