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. 2012 Jun 8;7(6):e38516. doi: 10.1371/journal.pone.0038516

Figure 1. Tergite phenotype caused by ubiquitous expression of CAG or CUG repeat RNA in Drosophila.

Figure 1

A, wild-type flies show a regular arrangement of tergite bands along the dorsal abdomen (arrows). B, An example of the disrupted phenotype, whereby tergites do not fuse at all (white arrowheads), or fuse only partially (grey arrowheads). C, Phenotype severity was scored on a scale of 1–4, images show typical examples from each category, where category 1 is like wildtype; category 2, tergites disrupted but not split; category 3, one tergite split; and category 4, two or more tergites split. D-E, Graphs showing the proportion of progeny within each scoring category. D, da-GAL4 driven expression of the EV control line (n = 161) and, E, da-GAL4 driven expression of 4xrCAA∼100 [line 1] (n = 148) gives no phenotype with almost all progeny like wild-type. F, da-GAL4 driven expression of 4xrCUG∼100 [line 1] (n = 271) or G, 4xrCAG∼100 [line 2] (n = 343) gives a tergite disruption phenotype. H, Schematic (not to scale) showing the location of histoblast cells (black). Histoblasts proliferate and migrate to form the tergite bands (arrows). I, Expression within the histoblasts using T155-GAL4 gives wild-type tergites in EV control progeny (n = 56). J, T155-GAL4 expression of 4xrCAG∼100 [line 1] (n = 25) gives a mild tergite phenotype, ***p<0.001 comparing the proportion with a phenotype in I and J.