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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: J Neurochem. 2012 Apr 19;121(6):881–890. doi: 10.1111/j.1471-4159.2012.07740.x

Figure 5. Screening of MOR256-17 with an expanded panel of odorants.

Figure 5

A) An oocyte expressing MOR256-17, Gαolf and CFTR is screened with 15 sec applications of 1mM IBMX, 0.006% DMSO (D), and 8 mixtures containing a total of 155 odorants. Each mixture contains 17 – 20 odorants, each at a concentration of 30 μM (the odorant composition of each mixture is listed in the Supplemental Table 3). B) An oocyte expressing a MOR256-17, Gαolf and CFTR is challenged with 15 sec applications of 1mM IBMX, 0.006% DMSO, and 4 sub-mixtures derived from the mixture 8. M8S1 contains geraniol, carvacrol, (S)-(−)-perillyl alcohol, thymol and 2-methyl pentanal. M8S2 contains o-methoxycinnamaldehyde, trans-3-hexenoic acid, benzyl cinnamate, ethyl butyrate and hexyl isobutyrate. M8S3 contains p-tolyl acetate, isopropyl tiglate, terpinyl formate, 1,4-cineole and dimethyl anthranilate. M8S4 contains indole, 6-methyl-5-hepten-2-one, allyl sulfide and γ-terpinene. 2-heptanone (HEP) tested separately. Each odorant was tested at a concentration of 30 μM. C) An oocyte expressing a MOR256-17, Gαolf and CFTR is challenged with 15 sec applications of 1mM IBMX, 0.006% DMSO and 30 μM of each of some of the individual odorants from the M8S1, M8S2, and M8S4 sub-mixtures: o-methoxycinnamaldehyde (MCA), geraniol (GER), 2-methyl pentanal (MP), terpinyl formate (TF), allyl sulfide, 1,4-cineole (CA), dimethyl anthranilate (DA), indole (IND), and 6-methyl-5-hepten-2-one (MH).