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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: J Neurochem. 2012 Apr 27;121(6):985–995. doi: 10.1111/j.1471-4159.2012.07742.x

Figure 3.

Figure 3

A) Quantitative assessment of the preventive effect of NAC upon process shortening induced by 3-NP. Bars represent the mean ± SEM of process length for each condition. Veh, PBS. B) Left panel, Western blot probed with anti-CRMP-2 of homogenates from OLN-93 cells incubated with PBS in the absence (−) or presence (+) of 3-NP. Anti-CNP in the same membrane was used as a control of protein loading. Right panel, bars represent the mean ± SEM of the immunoreactivity of CRMP-2 expressed in arbitrary units (A.U.). C) Assessment of the lack of effect of roscovitine (Rosc) upon the process retraction induced by 3-NP in primary OLGs. Bars represent the mean ± SEM of OLG diameter in each condition. Vehicle (Veh), DMSO. D) Quantitative estimation of the effect of the ROCK inhibitor, Y27632, upon the shortening of process of primary OLGs elicited by 3-NP. Bars represent the mean ± SEM of OLG diameter. Note the complete prevention of process shortening by Y27632. Vehicle (Veh), PBS. E) Prevention of 3-NP-induced process retraction in OLN-93 cells by Y27632. Bars represent the mean ± SEM of process length. **p<0.001, one-way ANOVA.