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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Cancer Lett. 2012 Apr 1;323(1):29–40. doi: 10.1016/j.canlet.2012.03.031

Figure 4. Effect of Graviola extract in the motility, migration, and cytoskeleton of pancreatic cancer cells.

Figure 4

(A) Wound healing assay of FG/COLO357 PC cells after treatment with Graviola extract. Microscope images (40X) of the artificially created wound in PC cells monolayer were taken before (0hr) and after adding Graviola extract (24hr); (B) Migration of FG/COLO357 PC cells after treatment with Graviola extract. The number of cells that migrated through the 8μm pores of a polyethylene terephtalate (PET) membrane was quantified in 10 random fields. Data represent the mean value of migrating cells ± standard error of mean (*p-value = 0.0009; **p-value < 0.0001, compared to untreated control cells); (C) Actin filaments were analyzed by confocal microscopy by Rhodamine-anti-Phalloidin staining of FG/COLO357 cells after treatment with Graviola extract. Nucleus was stained with DAPI. Scale bars represent 20μm; (D) Microtubules were analyzed by confocal microscopy after FITC-anti-β Tubulin staining of FG/COLO357 cells after treatment with Graviola extract. Nucleus was stained with DAPI. Scale bars represent 20μm; (E) Expression of proteins related to migration/motility of PC cells after treatment with Graviola extract. Protein lysates (30μg) were resolved by 10% SDS-PAGE. β-actin was used as a loading control.