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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Parasite Immunol. 2012 Jul;34(7):372–382. doi: 10.1111/j.1365-3024.2012.01367.x

Figure 4. P. falciparum GPIs induce cytokine production mainly through TLR2-mediated signaling.

Figure 4

FL-DCs derived from bone marrow cells of WT mice and TLR2−/−, TLR4−/−, TLR9−/− and MyD88−/− mice were plated in 96-well plates (1 × 105 cells/well) and stimulated with indicated doses of GPIs coated gold particles in 200 μl of complete medium. Pam3CSK4 (TLR2 ligand, 10 ng/ml), LPS (TLR4 ligand, 100 ng/ml), and CpG (TLR9 ligand, 2 μg/ml) were used as control stimulants. The TNF-α and IL-12 secreted into the culture medium were analyzed using ELISA. The experiment was repeated two times and the mean values ± SD from one of the experiments are plotted. *, p <0.05; **, p <0.01; ***p <0.001; ns, p >0.05.