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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Biochim Biophys Acta. 2012 Mar 28;1819(7):657–666. doi: 10.1016/j.bbagrm.2012.03.004

Fig. 1. Chromatin cycle – transient interactions of glucocorticoid receptor (GR) with chromatin revealed by fluorescent recovery after photobleaching (FRAP) method.

Fig. 1

A) Fast recovery upon laser photobleaching of the GFP-tagged GR (GFP-GR) associated with its respective regulatory elements demonstrates the transient nature of these interactions. B) Even faster GFP-GR recovery is observed upon hormone withdrawal. C) GFP-GR recovery profile in the presence and absence of hormone reflect differential affinity of the hormone-bound and hormone-free receptor to its regulatory elements. D) Schematic representation of the transient, nevertheless slower exchange of the hormone-activated receptor with the regulatory elements is reflected in the reduced mobility of the FRAP curve in C. E) Hormone-free receptor loses its affinity to specific sites and is extremely mobile as demonstrated by its FRAP curve in C.