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. Author manuscript; available in PMC: 2013 Jun 29.
Published in final edited form as: Brain Res. 2012 May 2;1463:1–20. doi: 10.1016/j.brainres.2012.04.047

Figure 3. Time-course of the effect of GABA plus THP on surface expression of α4Fβ2δ in HEK-293 cells.

Figure 3

A, Representative confocal images (63X) show an increase in surface expression first detectable after 0.5 h exposure of HEK cells to GABA (10 μM) + THP (100 nM), Anti-FLAG, Alexa-488 F(ab′)2 (green); cell stain, DAPI (blue). Scale bar, 10 μm. B, Averaged data. Peak levels of expression are achieved at 48 h treatment. All timed treatments terminated at the conclusion of the 48 h incubation period (timeline inset, GT, GABA plus THP). Vehicle, 0.01% DMSO. ANOVA, F(4,20) = 71.4, P<0.0001. *P< 0.05 vs. 0 h, 0.25 h; **P<0.05 vs. other groups.