48 h treatment with 10 μM gaboxadol (GBX) increases surface expression of α4Fβ2δ significantly compared to vehicle (0.01% DMSO) A, Representative confocal images (63X) of α4FLAG immunolabeling; B, averaged data. 2° Antibody, Alexa-488 IgG; cell stain, TO-PRO-3; scale bar, 10 μm. t(22) = 6.6; *P<0.0001 vs. vehicle. C, D, Increased expression was correlated with increased whole cell current response to acutely applied GBX. C, Averaged data. D, Representative currents; Arrow, onset of agonist application. t(7) = 2; *P<0.05 vs. vehicle groups.