48 h treatment with β-alanine (β-Ala, 10 μM, 50 μM, 1 mM) increases surface expression of α4Fβ2δ compared to vehicle (0.01% DMSO). A, Representative confocal images (63X); B, Averaged data. 2° Antibody, Alexa-488 F(ab′)2 (green); cell stain, TOPRO- 3; scale bar, 10μm. ANOVA, F(2,24) = 27, P<0.0001. *P<0.05 vs. vehicle. C, D, Increased expression was correlated with increased whole cell current responses to acutely applied gaboxadol (GBX). C, Representative currents; Arrow, onset of agonist application; D, Averaged data. ANOVA, 100 nM GBX, F(3,31) = 6.4, P = 0.0017; 10 μM GBX, F(3,31) = 6.3, P = 0.0018. *P<0.05 vs. vehicle groups.