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. Author manuscript; available in PMC: 2013 Jun 29.
Published in final edited form as: Brain Res. 2012 May 2;1463:1–20. doi: 10.1016/j.brainres.2012.04.047

Figure 8. 48 h Gaboxadol treatment increases gaboxadol responsiveness of cultured neurons.

Figure 8

A, Representative trace, tight seal, cell-attached current clamp recording of a cultured neuron (8 d) transfected with α4F, β2 and δ (48 h). Gaboxadol (GBX) produced an upward deflection, reflecting a depolarizing response. (Representative of 5 cells.) B, Representative currents, whole cell voltage clamp recordings of responses to acutely applied GBX (100 nM, 10 μM). Neurons were treated with vehicle or 10 μM GBX for 48 h. C, Concentration-response curves of averaged data. 48 h GBX treatment increased the maximum response and shifted the curve to the left (EC50, Con, 6.0 (1.71, 21) μM; GBX, 0.075 (.023, .248) μM). Dashed line, maximum GABA response indicated for each group determined with the response to a saturating concentration of GABA (100 μM). t(19) = 4.8; *P<0.0001.