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ECH0825 is required for E. chaffeensis infection.
Antibody against rECH0825, or normal rabbit IgG, was delivered to E. chaffeensis–infected THP-1 cells at 1 day p.i. by Chariot transfection reagent. A. Goat anti-rabbit conjugated with AF555 (red) was used to detect the delivered antibodies. Merge w/PC, image merged with phase contrast. B. Bacterial infection level was determined at 1 day post-delivery by western blot analysis with rabbit anti-P28 (E. chaffeensis major surface antigen).
