Ectopically expressed ECH0825 inhibits etoposide-induced apoptosis.
A. Double fluorescence labeling of pEGFP-N1- or pECH0825-transfected RF/6A cells treated with etoposide (100 μM) for 1 day; cells were labeled for DNA with DAPI (blue) and rabbit anti-ECH0825 (ECH0825; AF488, green). The white dashed lines denote contours of host cells. Scale bar: 15 μm.
B. Percentage of apoptotic cells with fragmented nuclei among pECH0825- or pEGFPN1-transfected cells. Data are presented as the mean ± standard deviation of three independent experiments. **Significantly different by Student’s t-test (p < 0.01).