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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Cell Microbiol. 2012 Mar 12;14(7):1037–1050. doi: 10.1111/j.1462-5822.2012.01775.x

Fig. 7.

Fig. 7

Ectopically expressed ECH0825 inhibits etoposide-induced Bax clumping, loss of mitochondrial membrane potential, and cytochrome c release.

Bax clumping (A, B), loss of mitochondrial membrane potential (C, D), and cytochrome c release (E, F) were inhibited in pECH0825-transfected cells compared to pEGFP-N1-transfected cells after 1 day of treatment with 100 μM etoposide. DAPI was used to label DNA (blue and gray); Bax was labeled with mouse anti-Bax and AF488-conjugated goat anti-mouse IgG; MitoTracker Red was used to stain mitochondria that had a high membrane potential. cytochrome c was labeled with mouse anti-cytochrome c and AF488-conjugated goat anti-mouse IgG; The white dashed lines denote contours of host cells. Scale bar, 15 μm. Percentage of cells with Bax clumping, loss of mitochondrial membrane potential, and cytochrome c release are shown in B, D, and F, representative data for which are presented as the mean ± standard deviation (n = 3) of two (D, F) or three (B) independent experiments. *Significantly different by Student’s t-test (p < 0.05).