Figure 1. Subnuclear localization of DFz2C and LamC at larval muscle nuclei and defective NMJs in lamC mutants (also see SF1).

A- LamC and DFz2C labeling (deconvolved) of muscle nucleus containing a DFz2C/LamC focus (box; enlarged in right panels) localized to the nuclear periphery (arrowhead in XZ plane). Arrows=DFz2C granule within the LamC framework-like structure.

B- Number of DFz2C and LamC foci/nucleus. N (same order as in graph)=450, 413, 302, 530, 328, 617, 593.

C- Localization of LamC-GFP and wild type LamC in muscle nucleus from lamC^GFP-trap/+ in relationship to DFz2C (box; enlarged in right panels). Inset is the same nucleus but overexposed.

Calibration=5µm A (left), 2µm A and C (right), 7µm C (left). Images are single confocal slices.

D–I- Larval NMJsdouble labeled with antibodies to HRP and DLG in

• D,E- a wild type NMJ at (A) low and (B) high magnification,
• F, G- a lamC null mutant NMJ at (C) low and (D) high magnification, and
• H, I- an NMJ from a larva expressing LamC-RNAi in muscles (LamC-RNAi-muscle).

Arrowheads=ghost boutons. Calibration=30µm D, F, H; 12µm E, G, I.

J, K- Morphometric analysis of NMJs showing

• J- ghost boutons, and
• K- bouton number.

N (same order as in graph)= 10, 19, 16, 16.

L- Morphology of synaptic boutons in (top) wild type and (bottom) lamC mutant.

Calibration=12µm.

M–O- Electrophysiological analysis of larval NMJs showing

• M- mEJP frequency,
• N- mEJPs amplitude, and
• O- evoked EJP amplitude.

N (same order as in graph)=8, 8, 5, 5, 7.

P,Q- Larval NMJs labeled with HRP and DGluRIIA antibodies in

• (P) wild type and
• (Q) a larva expressing LamC-RNAi in muscles.

Calibration= 5 µm.

R,S- Morphometric analysis of GluRIIA clusters showing

• (R) GluRIIA label volume and
• (S) total intensity.
• ***= p< 0.0001; **= p<0.01; *= p<0.05. Bars in graphs indicate mean±SEM.